How is vaping simulated?
A vaping machine is used: it’s a system controlled by a software that applies an aspiration to the vaporizer according to a defined protocol.
The generated aerosol is collected in a medium subjected to the treatments and finally it’s analysed.
Toxicological and chemical analyzes are performed on the collected aerosol
How are the analyzes performed?
To perform the toxicological analyzes, the steam collected using the vaping machine is made to interact for 24 hours with a 3D structure of cells that reconstructs the structure of the human lungs where the inhaled gases come into contact with the blood system. To evaluate the safety of vaping products, the parameters related to cell viability, functional damage and inflammatory response are measured and evaluated.
Chemical testing are performed on the steam generated by the vaping machine, in order to seek and quantify many potentially dangerous chemical substances, including all those indicated by the strict European legislation (2014/40/EU). The results are compared to the standard exposure limits; in general, the lower the quantity detected, the more harmless is the analysed vapor.